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首页> 外文OA文献 >RsmC of Erwinia carotovora subsp. carotovora Negatively Controls Motility, Extracellular Protein Production, and Virulence by Binding FlhD and Modulating Transcriptional Activity of the Master Regulator, FlhDC▿
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RsmC of Erwinia carotovora subsp. carotovora Negatively Controls Motility, Extracellular Protein Production, and Virulence by Binding FlhD and Modulating Transcriptional Activity of the Master Regulator, FlhDC▿

机译：欧文氏菌亚种的RsmC。胡萝卜素通过结合FlhD和调节转录调控因子FlhDC负控制运动性，细胞外蛋白质的产生和毒力。

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摘要

RsmC and FlhDC are global regulators controlling extracellular proteins/enzymes, rsmB RNA, motility, and virulence of Erwinia carotovora subsp. carotovora. FlhDC, the master regulator of flagellar genes, controls these traits by positively regulating gacA, fliA, and rsmC and negatively regulating hexA. RsmC, on the other hand, is a negative regulator of extracellular proteins/enzymes, motility, and virulence since the deficiency of RsmC in FlhDC+ strain results in overproduction of extracellular proteins/enzymes, hypermotility, and hypervirulence. These phenotypes are abolished in an RsmC− FlhDC− double mutant. We show that RsmC interferes with FlhDC action. Indeed, the expression of all three targets (i.e., gacA, rsmC, and fliA) positively regulated in E. carotovora subsp. carotovora by FlhDC is inhibited by RsmC. RsmC also partly relieves the inhibition of hexA expression by FlhDC. The results of yeast two-hybrid analysis revealed that RsmC binds FlhD and FlhDC, but not FlhC. We propose that binding of RsmC with FlhD/FlhDC interferes with its regulatory functions and that RsmC acts as an anti-FlhD4FlhC2 factor. We document here for the first time that RsmC interferes with activation of fliA and motility in several members of the Enterobacteriaceae family. The extent of E. carotovora subsp. carotovora RsmC-mediated inhibition of FlhDC-dependent expression of fliA and motility varies depending upon enterobacterial species. The data presented here support the idea that differences in structural features in enterobacterial FlhD are responsible for differential susceptibility to E. carotovora subsp. carotovora RsmC action.
机译：RsmC和FlhDC是控制细胞外蛋白/酶，rsmB RNA，运动性和胡萝卜欧文氏菌亚种毒力的全局调节剂。胡萝卜。鞭毛基因的主要调控因子FlhDC通过正向调节gacA，fliA和rsmC以及负向调节hexA来控制这些性状。另一方面，RsmC是细胞外蛋白质/酶，运动性和毒力的负调节剂，因为FlhDC +菌株中RsmC的缺乏会导致细胞外蛋白质/酶的过度产生，运动性和高毒力。这些表型在RsmC- FlhDC-双突变体中被废除。我们显示RsmC干扰FlhDC动作。确实，所有三个靶标（即gacA，rsmC和fliA）的表达在carotovora亚种中正调控。 FlhDC产生的胡萝卜被RsmC抑制。 RsmC还部分缓解了FlhDC对hexA表达的抑制作用。酵母两杂交分析的结果表明，RsmC结合FlhD和FlhDC，但不结合FlhC。我们提出RsmC与FlhD / FlhDC的结合会干扰其调节功能，并且RsmC充当抗FlhD4FlhC2因子。我们在这里首次证明RsmC干扰了肠杆菌科家族成员中fliA的激活和运动。胡萝卜肠杆菌亚种的程度。胡萝卜杆菌RsmC介导的flhDC依赖的fliA和动力的抑制表达取决于肠细菌的种类。此处提供的数据支持这样一种观点，即肠道细菌FlhD中结构特征的差异是导致对胡萝卜杆菌亚种易感性不同的原因。胡萝卜素R​​smC作用。

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